Searching in the literature I've come across a large number of publications where one of the key residues in eso-alpha-sialidases is ASP 151. It is assumed to act as an acid-base catalyst, donating a proton to the substrate, althought this particular residue should be deprotonated in the human phisiological pH.
So why is it assumed to be a proton donor? Does the reproduction cycle of influenzaeviruses involve an acidification in certain steps?
Hi,
if the ASP residue is buried, its pKa may rise enough to become protonated at higher pH. Regarding the reproduction cycle of influenza, an acidification step is required for endossomal release.
Hi,
thank you for your kind answer, you've cleared pretty much any of my doubts!
Actually, eso-alpha-sialidases fullfill their role during the endossomal release, so it's actually probable that ASP 151 is protonated.
- Badges
- Science topic
- Similar topics
- Linguistics
- Composition Studies
- Publications