22 September 2015 5 7K Report

I did a total RNA pico for mammalian cells and obtained the attached profile. The RIN value is 8.9, but the 18S peak is higher than the 28 S peak. Does this mean contamination? Or degradation of the RNA? Some people say that the 28S RNA in not very stable after the 2 mins denaturation at 70 degrees.

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