Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to use bag of words for action prediction?

Hi everyone, I'm working on an action prediction (i.e., early recognition) using Bag-of-Words. I know how to use BOW, but regarding action prediction, there is a subtle problem. In the...

19 February 2021 1,442 4 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

How can I make a center of mass vs time graph?

I have a trajectory file of about 1000 frames which I have calculated a center of mass for each frame. I would like to use the first frame as a reference and see how much the center of mass...

04 February 2021 5,774 1 View

Can people point me to interpretative phenomenological analysis (IPA) studies where there are only two participants?

I am in search of studies with a small n to support recent work completed in-depth with two participants regarding international students in Canada. I am not sure if framing things as two case...

23 January 2021 8,564 7 View

How to calculate the electric field in the rotating frame (the question in the general relativity)?

In the classical electrodynamics, there is one problem - to find the EM fields created by the charge moving with the constant velocity in the circular orbit or in the helical path (free electrons...

12 January 2021 3,689 1 View

How can I indicate to you that I am in fact the author of a book that you have informed me about for quoting other work of mine?

The question concerns this title: Viktor Smith Naming and Framing: Understanding the Power of Words across Disciplines, Domains, and Modalities. Routledge. All the best, Viktor

01 January 2021 4,197 1 View

Is it necessary to modeling detail of connection in opensees while analyzing steel moment resisting frames?

Dear all I want to model several steel moment resisting frames in opensees and my desire output after applying earthquake records, is interstory drift angles; i just considered rigidity of my...

14 December 2020 9,418 2 View

Does high correlation between features always mean similarity in information?

If two or more columns are highly correlated in a data frame, Is it always true that they will convey similar meaning and hence one of them is useless and should be dropped? How does this change...

02 December 2020 7,434 3 View

How to perform intention-to-treat analysis for repeated measures data in SPSS?

25 November 2020 6,281 3 View