I am running a concentration-response curve in vitro for an agonist of a G-coupled protein receptor.
The EC50 values I am getting are tens of thousands of times greater than those reported in the literature using the same assay, although these reports were in different cell types.
What factors could be contributing to the reduced potency?
I understand that reduced receptor density could increase EC50 but is that sufficient to explain such a different EC50? Are there any other reasons I am getting such a low potency? Is this level of variation typical between different systems?
(I tested the purity of my agonist to confirm it had not degraded).
Don't know what the effect is that you're measuring, but couldn't it just be that your cells react differently to the receptor activation compared to the other cell types?
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies