I have determined serum specific IgE reactivity to wasp venom of 30 clinically confirmed anaphylaxis patients using phadia set up and immunoblots. 12 of 30 had positive level of detection for phadia immunoCAPs whilst all of 30 had IgE reactive bands in immunoblots. How is that possible? Why is low sensitivity associate with Phadia CAP for wasp venom?
Maybe the other 18 patients just are sensitized to the bee venom...? Did you try to check it with Phadia...? And what about SPT and/or intradermal skin tests.. ? Are they compatible each with other ?
Thank you Dr Wojciech Feleszko
These patients are stung by Vespa affinis, a wasp native to Sri Lanka, and the caps are made of Vespular vulgaris. the Western wasp, venom. Patients have identified the specimens. Unfortunately, we could not perform skin prick or intradermal as the pure venom of Vespa affinis is not available. In immunoblots there were only one IgE band for bee venom which identified as hyaluronidase where in blots with Vespa venom resulted 5 bands in all these patients sera. That has shown the patients are truly stung by wasp. Do you have any idea to explain this?
Regards
Peshala
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