Can you explain your question further? Do you want to know what will happen if lambda max is not selected?
Mr. Aslam,
What you have reflected in mind under ''multiple reader''? Audience? If so, then, the answer to your question is: Because of, there is significant error contribution to the lmax values depending on spectroscopic method or algorithms used to process spectroscopic patterns or both of these.
Therefore, the data could not be reliable from perspective of chemometrics; for instance, when there is used UV-VIS spectroscopy. Their employment in property of evidence is questionable, because of there can be achieved only academic accuracy of results, but not legislation one. Among available vibrational spectroscopic methods, only FT-IR spectroscopy operating, however, explicitly, in absorption mode could be used to such purposes. However, this depends, strongy, on many experimental factors and parameters as well as methods for data processing of the IR-patterns.
during lab we made different tartrazine concentrations and put them in
Multiplate reader in lab and got absorption values. although we didn't choose lamda max in it while drawing the absorption spectrum and didn't select it either. my question is why don't we choose a lamda max while using multiple reding for absorption values and drawing a standard curve? Udaya Jayasundara
A Beer-Lambert plot will work at any wavelength - provided that there is some absorbance from the substance under test. Lambda max is usually choosen as it the best point if the light is not very monchromatic (large slit width).
Somettimes if the absorbance is very high (at lambda max) it is a good idea to offset the measurement as the absorbance reading around 0.3 is the most accurate.
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