I have visited many labs in different universities all over the world but only a few researchers know how to run QRT-PCR! What do you think?
I disagree. This is a very commonly used procedure and everything can come basically all in commercial kit form. You just need a real-time PCR thermal cycler in addition.
Gordon L Warren How about primer design for cDNA and choosing a reference gene, on the other hand, the calculations! all these complicated procedures you think an inexperienced person can do it even with a commercial kit?! in my opinion those are the reasons
Most primers are commercially available. There is software available to help you choose your reference gene(s) (e.g., Normfinder, RefFinder). A large fraction of your calculations can often be done by your real-time PCR thermal cycler software. I never implied that an inexperienced person can do this all by him- or her-self with no training. I just implied that qRT-PCR is not that hard to do any more and is commonly done. At least 3 labs on my floor in my building do the technique routinely.
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