I Michael

It could due to the fact that you have loaded the spectrum in a wrong way. When you load into a CARA project you have to pay attention to the Dimz Cinept and Dimy CTOCSY dimensions. They depend on how you have acquired the spectrum but usually the inept dimension is the D2 and the CTOCSY is D3, so they have to be inverted with respect to the default.

Then you have to open the spectrum with System Scope rotated.

Dim x is Cinept; 

Dimy is CTOCSY

and DimZ is H

I do not know if I clearly explain , let me know if you solve

Francesca

Hi Francesca,

thank you for the answer. I already figured that out in the meantime, it was exactly the issue that you explained.

Maybe you can tell me whether I am doing the assignment correctly now:

I am using the shown strip to assign a Ha signal which belongs to certain Ca (which aligns nicely with data from my 15N NOESY and my H(CCCO)NH), after that I execute the  "show depth" function and can find sidechain carbons.

Best,

Michael