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Questions related from Michael Reiter
Hi all, I just tried to find a list of all commonly used biological cofactors (NAD/NADH, PQQ/PQQH2, etc.) and their associated redox potentials but can't seem to find anything. Does anyone know of...
22 February 2023 6,856 0 View
Hi all, I can't seem to find a reference that describes the temperature range at which Komagataelle phaffii/Pichia pastoris can grow on methanol. Does anyone know and ideally have a reference...
10 December 2022 8,386 0 View
Hi all, I want to access the sequence listing of a certain patent. However, it seems that there is no direct way to access the file referenced in the patent. How do I proceed in such a...
27 July 2019 10,024 3 View
Hi, I would like to generate a 1 molar (or at least 0.1 M) solution of geranyl pyrophosphate from GPP Lithium salt (http://www.sigmaaldrich.com/catalog/product/sigma/76532?lang=en®ion=US)....
01 March 2017 1,060 3 View
Hi, I want to get an idea of the structure of a protein which structure has not been determined as of now. However, I know of a protein that shares two conserved amino acid motifs, which I want to...
30 January 2017 7,699 4 View
Hi everyone, I am currently doing the sidechain assignment for my protein of interest, but I am stuck. I processed my spectrum with NMRPIPE and in nmrDraw it looks fine. However, when I load it...
26 August 2014 6,967 2 View
I am producing two proteins with E. Coli. One is fused to a his-tag, the other one to a Thioredoxin tag. Both can be cleaved off with TEV-protease. When I run a gel after purification, I can...
14 June 2014 4,141 17 View
Hi all, with the rise of CRISPR there has been a wave of genomic modification techniques. In your opinion and judging from your experience what is the best (i.e. highest efficiency, fastest),...
01 January 1970 4,127 1 View
