I measured an intact protein with ESI Ion Trap and got 3 ions with multiple charges. I focussed on the one with the highest relative abundance.
I did a full scan with SIM and only a SIM.
The area is much higher for the full scan + SIM.
Why is this better for quantification?
The are is given for TIC or only for one extracted ion?
Normally SIM gives better sensitivity.
I have both areas. So a TIC with the area and a SIM with an area for my extracted ion.
On the left only SIM and on the right full scan followed by SIM (one method).
Yes, the area is bigger, but check the Signal to Noise ratio for this peaks (besides this peak looks to be overloaded and tailing). Probably better value will be obtained for SIM - it even looks like that from the image. You don't need such a big area for quantification (you can also compare the RSD values of peak areas for each setting for a sequence of 3 injections), but if you will have very low concentrations, the SIM still will give the answer much above the noise level comparing to SCAN.
regards,
G
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