Why influenza virus HA gene gives faint band in PCR generally ?

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Can any one tell how to amplify polymerase gene of influenza A virus using universal hoffman primers with details?

I followed Hoffman article with annealing temperature 58 with Taq polymerase and as well as Mastermix. It doesnt amplify

11 December 2018 6,085 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

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Low-yield gel extraction problem?

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Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Andrew Ting

Well, there are a few things you can do to get good yield.

1) Firstly, check your the design of your primer

2) Then, you need to optimize your primer's annealing temperature

3) Concentration of your reagents in your PCR mix should be enough

4) You can also increase the number of cycles.

Good luck

Martin Chenal

5) You can scale up your reaction, up to a final volume of 50ul.