Why influenza virus HA gene gives faint band in PCR generally ?

More Sivasankar Panickan's questions See All

Can any one tell how to amplify polymerase gene of influenza A virus using universal hoffman primers with details?

I followed Hoffman article with annealing temperature 58 with Taq polymerase and as well as Mastermix. It doesnt amplify

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How do I increase the yield of my PCR product?

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Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to address this issue in the analysis of Real-Time PCR results?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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How vaccines of covid 19 work?

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Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

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MiRNA qpcr problem?

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How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Andrew Ting

Well, there are a few things you can do to get good yield.

1) Firstly, check your the design of your primer

2) Then, you need to optimize your primer's annealing temperature

3) Concentration of your reagents in your PCR mix should be enough

4) You can also increase the number of cycles.

Good luck