What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Could bone tissue be saved in 70% EtOH after decalcification before processing?

I got the mouse tibia and femur tissue harvested, fixed with formalin 48h and decalcified with 14% EDTA 72h before paraffin embedding. This protocol works well for both HE and IHC. Now, for the...

18 October 2020 5,318 1 View

Why are G-quadruplexes a thing? Why not A, T, or C-quadruplex?

Guanine-rich DNA sequences can fold into four-stranded, noncanonical secondary structures called G-quadruplexes (G4s). G4s were initially considered a structural curiosity, but recent evidence...

27 September 2020 9,105 6 View

Could someone provide the cif file of classic organic salt/organic cocrystal TTF-TCNQ?

27 September 2020 178 3 View

How does methylene blue fluoresce under dark UV?

Will it show as blue color under dark UV light (say 390 nm to 400 nm range) ? Does it degrade over time under long exposure of UV light (i.e. losing its fluorescein characters) ? What...

23 September 2020 9,752 2 View

My citrate buffer for IHC turned light yellow after boilded 30min with tissue slice. Is it normal?

My citrate buffer for IHC turned light yellow after boilded 30min with tissue slice. I have adjuct the PH to 6 and buffer was fresh made. I just wondering why it turned yellow after boiled with...

09 September 2020 5,357 1 View

Can I only use Matlab-Simulink to model the network consensus problem of a multi-agent system in DC micro-grids?

Hello everyone,recently I have met some problems in my study.I want to achieve a multi-agent system in DC micro-grids,but I never used java so that I can't use Jade to achieve it. Can I only use...

07 September 2020 6,342 2 View

How to avoid washing cells out during the changing medium step?

I'm Culturing adult mice cardiomyoctes and during changing medium step the cells seems washed away. Any tips about how to avoid loosing cells during washing step?

02 September 2020 4,788 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Should I use Pressure Based Solver or a Density-Based Solver?

Dear researchers, I want to simulate a flow (air) in a wind tunnel, and I want to integrate a fluidic actuator inside it in order to control the separation of the boundary layer. At the blower...

22 February 2021 4,882 3 View

No title

17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to Measure Consumer Willingness To Change from petroleum-fueled vehicle to electric vehicle?

I wanted to calculate the probability of one consumer shifting from using a petroleum-fueled vehicle to an electric vehicle. Since my background is in engineering I do not know much about how to...

15 February 2021 9,190 5 View

How to apply a voltage sinusoidal/harmonic load in ANSYS APDL?

Hello all, I want to simulate a Dielectric Elastomer Actuator DAE (an electrostatic-Structural analysis). I want to apply various voltages and frequencies into the DEA in the form of sine wave....

13 February 2021 3,261 3 View

How to extract the yearly cash flow data (not time-series) from HomerPro in Excel? Also how to extract the monthly average electric production data?

I want to plot the monthly average electric production data and cash flow data in another software to get a more clear graph. For this, I need to extract the monthly or yearly data and not the...

11 February 2021 2,740 2 View