I am trying to perform same experiment on my magnetic nanoparticles, but found some uncommon results in scanning in different directions. scanning from right to left and from left to right, we see a change in contrast of image, i.e. black and white. It seems like a shadow that appears all the time on all our particles. Can I ask how should I manage to solve the issue. more generally is it an artifact or this has a meaning and should be interpreted?

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