Hi everyone!
I would like to ask for suggestion to improve the quality of my SEM image. I tried to analyze bacteria using Phenom SEM (a benchtop unit). For the sample preparation, I fixated the bacteria using 2% glutaraldehyde in 0.1 M potassium phosphate buffer pH 7.2, then washing using 1x PBS solution. Followed by dehydration through a graded ethanol series.
I've attached one of the SEM images I obtained. I would really appreciate any advice or tips to enhance the image quality, particularly regarding sample preparation or instrument settings.
Thank you in advance for your help!
Your image is out of focus (nothing to do with specimen preparation). Check if your Phenom SEM is capable to provide x17k with some nice conducting specimen. Also contrast of your picture was too low, but it is secondary, not important mistake.
Was the sample coated with metal for conductivity? It seems not since you are using 1 Pa of atmosphere and BSE imaging.
I concur with Vladimir Dusevich, how does a normal sample look under similar conditions? What gun does that SEM use? If it is a W gun, the image may be about the best you can do.
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