Hyunjin Kim

5 Questions 1 Answers 0 Followers

Questions related from Hyunjin Kim

How to interpret and use Rosetta dG_separated in binder design filtering?

Hello, I am currently designing a protein binder targeting a kinase, and I’m using Rosetta for structure-based filtering. I received a PDB template binder from my professor and have been...

08 July 2025 6,971 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

07 August 2024 5,951 2 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

21 July 2024 5,237 1 View

What should I keep in mind when designing a gene construct when trying to co-transform to increase protein expression?

I am having trouble expressing a protein and have heard that co-transforming it can improve stability and increase expression, so I am looking to try this experiment. I only need the kinase domain...

09 July 2024 2,979 5 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

01 July 2024 6,911 5 View