Why i am facing fungal contamination problem in hairy root culture?

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Hayat Essalmani

Add an antifungic in the culture medium

Prosanta Kumar Dash

Hello Maliha!

Possible reasons of fungal contamination in MS media could be-

1. Improperly sterilized media and/equipments.

2. Defect of air filter of laminar flow.

3. Movement of peoples behind you when you worked under hood.

4. No mask on your mouth while you inoculated.

Please double check the above mentioned possible reasons of contamination.

Maliha akbar Soomro

Hayat Essalmani Thank you for reply please refer any antifungic for MS media