Why i am facing fungal contamination problem in hairy root culture?

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Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

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What is the best surface to culture cells isolated from a skin biopsy?

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Difficulty in cloning of 1kb gene into vector?

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Prosanta Kumar Dash

Hello Maliha!

Possible reasons of fungal contamination in MS media could be-

1. Improperly sterilized media and/equipments.

2. Defect of air filter of laminar flow.

3. Movement of peoples behind you when you worked under hood.

4. No mask on your mouth while you inoculated.

Please double check the above mentioned possible reasons of contamination.