A paper in Science (Y. Roman-Leshkov, Science (80-. )., 2006, 312, 1933–1937.) conducted HPLC analysis for sample in methyl isobutyl ketone (MIBK) with mobile phase of 2:8 v/v Methanol:Water.
This condition is very wierd because MIBK is insoluble in aqueous phase.
However, the analysis seems to work well and many papers citing this paper also employ the same condition.
Does anyone know why HPLC can work with the immiscible combination of sample and mobile phase and what is happening in a column?
Hi Shusaku,
take a look to this article:
https://www.sciencedirect.com/science/article/pii/S0021967309002131
Regards
Markus
Hi Markus,
Thank you for telling me an interesting paper!
It was surprising but I'm convinced.
Thank you!
Shusaku
Quite simple, use a solvent that is misscible with both oil and water, for example ethanol. Check the tables of solvent properties.
Methanol or methanol-water mixtures probaly will work as well as demonstrated by the quoted paper.
It depends on the relative amount of lipofilic substance to be dissolved in the mobile phase.
Dear Jos,
Thank you for your practical advice!
I'll check the tables for my analysis.
Best,
Shusaku
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