Why Green tiger shrimp Penaeus semisulcatus is having more resistance/tolerance to white spot syndrome virus than black tiger shrimp Penaeus monodon?

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How is sperm alive in closed thelycum of female penaeid shrimp until shed off exoskeleton during next moulting ?

Penaeid shrimp physiologist/biologist

21 May 2015 7,147 7 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,112 1 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Does egg white interfere with any histology staining?

I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?

02 July 2024 8,261 0 View