In most of the research papers discussing diversity of endophytic fungi, molecular identification by ITS rDNA sequencing is usually followed by phylogenetic analysis. I am unable to understand what exacly is the importance of the analysis. I understand that as the name suggests it is done to understand phylogenetic relationships but how that is of any consequence in understanding the diversity of fungi or how to interpret the phylogentic tree is beyond my knowledge. Please help me understand.
Following ITS rDNA sequencing, phylogenetic analysis helps place fungal species within an evolutionary framework, offering insights into their evolutionary connections, origins, and diversity. By examining the tree, researchers may learn how different fungi are connected and develop a more thorough understanding of the structure and development of the fungal community.
The phylogenetic tree helps place a species in its family tree, revealing similarities and differences with other species. If you need guidance on creating a tree from the ITS sequence, feel free to email me, and I'll share some helpful video links.