Rice straw when subjected to Alkali pretreatment produced less cellulase than the unpretreated when Trichoderma was used for enzyme production under ssf.
One reason for the reduced cellulase production on alkali pretreated rice straw can be the problem with pH value of the SSF medium that can lead to decreased growth of Trichoderma and as the result, to the less number of hyphal tips as the major sites of growth and secretion of extracellular enzymes.
The basic condition can possibly inhibit the growth of enzyme. enzymes could be pH sensitive.
I am totally agreeing with Dr Babak Salamatinia. pH sensitivity is the vital cause.
After pre-treatment of the substrate with alkali you should have to neutralize that substrate again by washing the substrates with distilled water till it be near to 7.0 pH.
Or else it will affect the growth of fungi as we know fungi love to grow as acidic to neutral not much in alkaline conditions.
All the best.
-AKM
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