I have been using the jumpstart red taq polymerase mix from Sigma. I get a good amplification but the problem arising is that it starts degrading within 48 to 72 hours. Next time I'll purify the PCR product but can you think of possible reasons for degradation because there is no issue about contamination of tips etc.?
Hi Jaya,
PCR degradation normally donot occur due to contamination, but there are examples for such as well. I would like to ask you as to what temperature do you store the product? If you store it at 4'C, then the product will be reusable for a week or two; but I guess it will not be optimum for the cloning experiments, if any. In that case, I would suggest you use -20'C preferable. It gives a good yield and preservation potential as well. All the best.
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