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I have been using the jumpstart red taq polymerase mix from Sigma. I get a good amplification but the problem arising is that it starts degrading within 48 to 72 hours. Next time I'll purify the...
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A PCR reaction resulted in amplification of the desired gene but also gave two non-specific DNA bands. I want to cut the band of my interest, extract the DNA and amplify it again. When i used an...
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