Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

No title

Good morning, dear community, I have a preliminary report from an in silico analysis of non-coding short sequences against the SARS-CoV-2 virus. I'd like some advice about which indexed journals...

18 July 2020 5,365 1 View

Mask for outdoor exercise during covid pandemics?

Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...

08 July 2020 4,927 2 View

Help editing citantion style in Mendeley. Multiple paper by the same authors?

I'm trying to edit an existing citation style to suit my needs (Journal of Tropical Ecology). I edited it so the inline citations go from newest to oldest reference (Smith et al 2019, Jones et al...

15 June 2020 9,926 4 View

How to measure ratio/amount of PCR products?

Hello, I am trying to look at the efficiency at which a Gene is cut after tamoximen treatment. Thus we got a set of primers that can tell us weather the Gene is cut or not. We wanted to measure...

02 February 2020 7,599 3 View

Why GFP PCR in E. Coli is not about 50% 50% with respect of GFP expressing bacteria and non-GFP expresing bacteria?

As you can see, most of our colonies express GFP while we expected to get only 50% of these or less.

04 December 2017 8,378 3 View

Bumpy melting curves in qrtpcr? I am having some extra picks in my melting curves. I think is primer dimer since it is in NTC?

I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...

05 November 2017 5,899 7 View

What causes smearing in western blots?

I ran a 15ug western blot using protein from treated mammalian cells. The top bands are Ligase 4 and the bottom bands are B-Actin. I am not sure why there is smearing in the Ligase 4 bands but not...

12 September 2017 3,730 2 View

Whole cell fatty acids from hydrocarbon soil samples

05 June 2017 6,186 0 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Am i able to conduct a factor analysis on each of my subscales, as opposed to the whole scale?

My psychometric scale contains 38 items in total, with 4 subscales (20, 6, 6 & 6 items respectively? Can i run a factor analysis for each subscale rather than the scale as a whole for the...

24 February 2021 8,856 8 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View