Why does the MAF command in Plink software eliminate all SNP?

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Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

What are the prices for the following antibodies?

BD Pharmingen™ FITC Mouse Anti-Rat αβ T-Cell Receptor (561672) BD Pharmingen™ APC Mouse Anti-Rat CD4 (550057) BD Horizon™ V450 Rat anti-Mouse CD8a (560469) BD Pharmingen™ PE Mouse Anti-Rat CD45R...

10 May 2024 3,072 0 View

What is the recommended sample size for the validation of a standard questionnaire consisting of 40 items?

Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...

26 March 2024 1,660 11 View

Why does a precipitate form rapidly when i add a solution (Nitric acid + water ) to a solution of TTIP in isopropanol to ?

So I have ben trying to prepare TiO2 nanoparticles but i am getting trouble with the fromation of White PPT which later dissolves to form a clear solution that unfortunately doesnt form a gel. it...

25 March 2024 8,808 2 View

Does antigen retrieval for IF on tissue samples need to be done on the same day?

For both heat-induced or proteolytic-induced antigen retrieval, does the AR needs to be done on the same day of the IF? Or can be done one day in advance, store the slides at 4º, and performe the...

06 March 2024 220 1 View

Any empirical study about theater education for immigrant students?

03 March 2024 6,134 0 View

How can I model a packer in Abaqus for segmental tunnel linigns?

Currently, I am trying to model packers made from recycled plastic material (E=360N/mm^2 and a poisson ratio of 0.4). The best way to model these packers would be to only adjust the contact...

26 February 2024 5,699 1 View

What is the pH and osmolarity to measure the activity of myeloperoxidase (MPO) from the formation of hypochlorous acid?

Optimal buffer and conditions to determine the enzymatic activity of MPO by the colorimetric method that is based on the formation of hypochlrous acid which reacts with taurine to form the...

07 February 2024 3,056 0 View

Can convergence (SCF) be improved for transitional metals?

In Guassian, which convergence algorithm is best for working with transition metal interaction? It is taking a long time to even get the first block of coordinates, in the output file it said to...

28 January 2024 4,276 1 View

What is the ideal microscope objective (20x or 40x) to assess the size of adipocytes in abdominal adipose tissue in a murine model?

I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...

09 January 2024 7,149 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View