How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What kind of detector is used in the double slit experiment to determine through which slit the photon or electron passes?

Explain exactly what the detector does and if the choice of detector affects the outcome. Einstein and Bohr had some discussions on this. What's the current situation?

29 December 2020 1,984 5 View

Interpreting and troubleshooting anomalous HPLC results?

I am running a simple isocratic Shimadzu HPLC system coupled to a fluorescence detector to detect and quantify biogenic amines from in vivo samples. But my results are not as expected and I'm...

10 November 2020 7,027 5 View

What HPLC detector to choose to detect surfactants?

Colleagues We have a Waters e2695 HPLC instrument (with 2489 Uv-Vis detector). Recently we initiated the analysis of surfactants/lipids, which are unfortunately poorly visible to UV detector. We...

08 November 2020 4,645 18 View

Can anyone suggest a cause for a lack of fluorescent signal from a Diorex UltiMate 3000 RS detector in an HPLC experiment?

I am trying to detect viral capsids using an UltiMate HPLC system. I have a strong peak at the expected retention time using a 260/280nm UV detector, however I am not receiving any signal from the...

27 October 2020 3,786 2 View

What detector/sensor would be the best to use in measuring size of plastic in bodies of water?

We plan to identify the sizes of plastic in bodies of water from the surface using a device, but we are not so sure what to use best in order for us to identify it. Should we use infrared sensors,...

07 October 2020 2,702 6 View

Can I use Ultrs AQ C18 column for aflatoxin ?

07 October 2020 9,811 2 View

Does anybody have a method for quantification of neomycin sulphate using HPLC with a UV/Vis detector& reversed phase columns?

Does anybody have a method for quantification of neomycin sulphate using HPLC with a UV/Vis detector& reversed phase columns?

04 October 2020 6,181 3 View

Why is the transmittance measured by FTIR greater than 100%?

26 September 2020 4,036 1 View

Why won't my Perkin-Elmer Clarus-500 GC save my data?

Greetings. Our lab has a Perkin-Elmer Clarus 500 GC equipped with an autosampler and an electron capture detector (ECD). We run the instrument using the TotalChrom software (also called TCNav)....

22 September 2020 6,337 2 View

For Pump - Probe:how to test?

Recently we have built a pump probe system, here is our experimental light path diagram. We added a silicon-based probe detector in front of the beam splitter to detect the optical power entering...

07 September 2020 2,068 1 View