I think you could construct a fluoresence report gene on the plamid, and do you check the protein expression of this gene, if above could not get a good results, then you will consider change a tansfection method.

Xiaoyun Li Hello, I just found that my gene, MZF1, that I knocked down regulates gapdh levels (the control gene). Therefore literature suggested not using Gapdh as a viable control gene. Do you know if researchers use any other house keeping gene in that case?

Maybe you could try beta actin or beta tubulin