Transformation of Bacillus subtilis?

Colonies appeared after transformation of pHT01 plasmid into Bacillus subtilis, and band was confirmed by performing colony PCR. However, if the colony is picked and grown (in LB+antibiotics) and...

27 October 2020 609 4 View

Is this correct molecular docking?

Hello, buds I need your help to interpret the attached data. R group in serine is CH2OH, but the data show the two O in the group with hydrogen bonds with a molecule. Is this correct?...

02 October 2020 7,787 6 View

Are there any corporations that have launched Enterprise MOOCs to the public?

I am studying Enterprise MOOCs and can't seem to locate very many empirical articles for this area. The model I am interested in is similar to openSAP (https://open.sap.com). Please share any...

28 September 2020 6,813 2 View

Dataset size and the need for cross-validation (medical domain)?

I am currently envisioning a deep-learning model that classifies pulse waveforms (normal vs. pathologic). At this time, the no. of pulse dataset is about 2,000,000, and the balance of normal and...

31 July 2020 6,713 3 View

[ELI5] FRET assay, Protease assay, Michelis Menten. How would you convert the fluorescence signal to the velocity (concentrion/time)?

Hello i am fairly new to the field of physical chemistry and only have a vague memory of it from undergrad classes. my FRET assay is set up like a regular FRET assay, so the enzymetic activity of...

18 June 2020 9,730 4 View

Do buffers need to match for DNA (aptamer) to protein binding in SPR on NTA chip?

Hello, As a newbie to SPR, I've been having problems with optimizing aptamer-protein binding on an NTA chip. My protein has a his-tag on it. Any help would be greatly appreciated. Question 1:...

14 June 2020 4,586 3 View

How can I interpret this SEM image?

This SEM images are Li doped Cu2O thin films. Precursor of Li is LiCl(Lithium Chloride). I think there is no crystallinity. Is this correct?

31 July 2019 7,787 4 View

How can we calculate the dead cells in a specific population of Candida cells ?

How can we calculate the exact number of the dead cells in a specific population of Candida albicans cells, with or without staining ? If a dye were used, which one is better ?

27 November 2018 4,432 3 View

Does anyone know the BRCA1 status of the prostate cancer cell line 22Rv1?

ATCC catalog number is CRL-2505

06 November 2018 5,697 3 View

Problem of urine LTE4 level in ELISA test?

14 October 2018 4,494 1 View

No title

02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View