Hi colleagues, I have Arabidopsis thaliana wild type (Columbia) seeds. Seeds germinate well and their seedlings with first leaves (green) develop 1-2 cm long. The first leaves of seedlings are beautiful and green. However, seedlings do not develop after this stage and they remain very weak. Although I have tried many ways (Rockwool, hydroponics, soil, Petri dishes with Hoagland or MS), the seedlings do not grow and are the same even after 21 days.
I wonder what the problem is.
Thank you in advance for your contribution
Check temperature, it should be 18-20 C. Also light should be 16 hour photoperiod. Also check substrate water and nutrient content for optimum levels
Try to controle temperature , photoperiod , intensity of the light, contener volume is also important for plant developpment
Apart from other environmental cues, its important to prevent exposure to relative humidity lower than 50% and lack of air exchange also prevent development of good leaves. We grown many of Arabidopsis accessions in small pots and prevented direct contact of leaves with the growing substrate using a black plastic sheet. When I grow them in rockwool or in vitro, the size of the leaf was considerably decreased. Have a look on the following paper:
Aliniaeifard S, van Meeteren U. 2014. Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning. Journal of Experimental Botany 65, 6529-6542.
Many thanks for your contributions.
My growth chamber is so fine, and I've arranged its conditions according to the growth of Arabidopsis. I will still consider these recommendations.
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