Hello,

maybe. Also could be due to dirty surface of transiluminator or optical lens of camera (contaminated by EtBr or SYBRgold), try to clean it by wet paper towel. Alternatively you can try to stain your gel after electrophoresis in 1x EtBr solution (50 ul 10 000x EtBr in 500 ml 1x TAE for 20 min) and destain in TAE buffer (30 min). Solution of EtBr can be used repeatedly for at least 1 month. But be careful and always wear nitril gloves, it is strong mutagen.

Good luck !

Martin

Hi

Have you tried moving your gel a side on the transilluminator surface? May be the UV lamp has a problem, may be you need to clean the surface from any remnants!

Good Luck

Thank you for the suggestions.