Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

Age of the Isimila Prehistoric Site in Tanzania?

Does anyone knows results from the new dating program from the Isimila Prehistoric Site in Tanzania?- of course without to give away too much, before a final publication. The data of ca 260...

03 February 2021 6,864 3 View

What if there is no population to pilot an adapted questionnaire? What about its reliability and validity?

Hello, I'm new to this platform, and this is my first ever post in here. So, please excuse me in advance if I happen to cause any inconvenience. Coming back the question; I have just adapted a...

26 January 2021 9,030 4 View

No title

Engineering geologists may know that the originators of the empirical (a posteriori) Q-system method of rock mass classification for single-shell tunnel and cavern support estimation, were Barton...

13 December 2020 5,103 3 View

Why the oral cancer cell line from metastatic tongue origin is showing less aggressive property?

I am working with oral cancer cell line SCC090 (ATCC). As per ATCC data sheet it is originated from metastatic tongue. It's primary site is not mentioned. Due to the origination from metastatic...

08 December 2020 5,674 3 View

Who created the NAMI Self-Care Inventory (SCI)?

The SCI questionnaire for measuring domains of self-care appears in several places on the internet and scantly in the literature. There are no published validation studies or...

02 December 2020 6,848 1 View

GLMM in SPSS?

30 November 2020 2,716 4 View

Can you help me with some books?

My field of research is the philosophy of physics and I look for Petrus Hoenen's books in English or some Romance language (French, Italian or other). This author originally edited in Latin at the...

16 November 2020 3,982 1 View

There seem to be many pro and con for Punctuated Equilibrium theory. What is the most recent answer?

As I know PE theory raised a typhoon for evolutionary scientists that time. The authors were proud of its novelty. I found, however, the punctuation giving abrupt speciation had originated from...

26 September 2020 2,936 3 View

How can we trace the origin of cancer at the cellular level?

I want to know how we can precisely trace the lineage of cancer progression. Is it originate from the mutation of a Normal cell or a Stem cell? Is there any method available to precisely delineate...

14 September 2020 7,493 3 View