When I load a protein sample, the sample drifts up the sides of the sample well.My Running Buffer is Tris-Tricine-Hepes system.And the sample strip will become U-shaped after starting to run.
Buffer component：
100mM Hepes
100mM Tris
100mM Tricine
0.1%SDS
If the sample is rising after you add it to the well, it is probably because it's density is lower than that of the upper reservoir running buffer. You should be able to fix this by increasing the glycerol concentration in the sample buffer.
Are you rinsing the wells out thoroughly just before loading the samples. Sometimes salts and acrylamide monomer leach out of the gel and form a high salt barrier in the well leading to poor sample running
That focussing of the sample towards the centre of the well can happen with samples containing too much salt. It might be interesting to see the result of loading half and quarter amounts of a sample of any dna sample in your loading dye in a future gel. Also be sure to thoroughly mix the sample and sample loading buffer before loading on the gel
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