Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What is the maximum wavelength and absorbance for dodecyl trimethylammonium bromide?

I am measuring the absorption of dodecyl trimethylammonium bromide surfactant using UV spectrometer. I am trying to set the wave length range for the experiment. Please does anyone have a idea of...

07 February 2021 7,720 1 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

How to synthesize pyrrolium ionic salt?

I am keenly interested in the synthesis of heteroaromatic ionic salts for use as an organic template. I want to synthesize alkyl pyrrolium chloride or bromide through a simple addition reaction...

30 December 2020 2,778 3 View

Is it unethical to compare racial groups who are LGBT?

Hello everyone, I am currently conducting an undergraduate thesis on the unique challenges faced by Middle Eastern LGBT individuals due to their cultural background (often includes traditional...

08 October 2020 8,163 4 View

Plasmid visualization on gel electrophoresis?

I extract the plasmid from e. Coli, on nano drop its concentration was 60.but no band was formed in gel electrophoresis?I use 1% agrose gel with 2.5 ethidum bromid.

06 September 2020 4,438 11 View

Is it dangerous to inhale Lithium bromide?

Hello all, I am diluting LiBr with water and I wonder if I should do it under the fume hood or if it is okay to do it anywhere in the laboratory. Then, if I might do the reaction under the fume...

21 July 2020 7,892 3 View

Do Carborane Acids react with Inorganic Salts?

I couldn't find any information or articles about it. Does anyone know if carborane acids react to these specific inorganic salts: chlorides, bromides, iodides, or thiocyanates? Please also tell...

06 June 2020 5,075 4 View

Can CTAB stored in a refrigerator still be used to create extraction buffers?

Hello, Our lab is working on DNA extraction. We are creating new solutions for a new protocol. Unfortunately when our main CTAB (hexadecyltrimethylammonium bromide) stock was stored from last...

13 February 2020 8,652 1 View

What is the significance of temperature on perovskite thin-films lattices and the band gap energy?

An experiment was conducted on perovskite thin films made up of methyl ammonium bromide and lead iodide.the films made were subjected to different temperature before measuring the band gap...

27 January 2020 552 1 View