I am trying to cocrystalize my protein with a sugar ligand, for this protein in particular I obtain only clusters, I tried to play around the pH( from 4,5 to 8,5) using the condition with clusters but nothing. I am wondering if I need to perform an SEC on my protein to get rid of some contaminants that can affect my crystallization. If you have any suggestion that can help me, I will be happy to try.
See answers to this question: https://www.researchgate.net/post/Why_are_my_protein_crystals_clustered_together
Read: Article Optimization of crystallization conditions for biological ma...
I would call this showers, rather than clusters,
If you haven't already SEC your protein before setting up trays, i would do.
Also, you may need to optimise the condition you have further to move the crystals from showers to clusters.
But, as Annemarie Honegger has linked to you can try to break off pieces, loop those individually to shoot as single crystals.
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