01 January 1970 0 5K Report

I transformed E coli JM109 with pMV361 (Kan resistance) and had a negative control (transformed with water) and 10 plates of the bacteria transformed with the plasmid. Those 10 plates have barely 1 or 2 colonies with the phenotype expected for E. coli, but the negaive control has over 7 colonies some of them kind of orange.

I would expect that if the plates were not correctly made, every one of them would be contaminated. But they have what we expected, very little grow, white, round.

Attached the picture of the negative control plate.

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