Why do i get RNA degradation when I extract it from Trichoderma in vitro. Note, I collect mycelium and directly put it in liquid nitrogen, but when i checked it i found all cell are degraded?
Hi Ali, I think you should check RNA isolation protocol. If your cell count is high, you should use more Trizol. On the other hand, for cell membrane disruption, do not use sonicator or use weak wave and do it shortly.
Good luck!
thank you my dear
may be your opinion is right.
in fact, i tried several times using any way without useful.
Do you have an idea of the typical size of Trizol relative to the amount of RNA used?
please
In general, using 1 mL Trizol for 50 mg tissue is enough. But the amount of trizol may change tissue to tissue. Also, it depends the cell type and other living things.
For instance, we should use more trizol for fatty tissues. I think you should try to use 20% more trizol for your sample.
Sincerely
I will try using different sizes from Trizol for my fungus tissue.
Thank you very much
Greetings and sincerity
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