I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally resuspended in 10% glycerol. When I attempt to electroporate, arcing occurs. This happens even when I use only the competent cells without the plasmid, indicating that it is not due to the presence of salts in the miniprep.
are you using new electroporation cuvettes? (they can be reused several times after extensive washing but they all finish one day in a flash!!) are you sure of your electroporation program? are you sure of the purity of your water (use autoclaved or filtered milliQ water 18.2 MΩ.cm) and glycerol ? maybe you have some salt in your water or glycerol... check their resistivity, do more washes... for E. coli we do not use glycerol just water...
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