Emilia Bottero

3 Questions 2 Answers 0 Followers

Questions related from Emilia Bottero

What are the possible effects of DTT or β-mercaptoethanol, on gDNA amplification?

I used the Intact Genomics FastAmp® Plant Direct PCR kit and when seeding the gel with the PCR product there was a lot of DTT smell. After running the gel, partially degraded dna was observed,...

11 April 2024 2,124 2 View

Why do I get an arc when I electroporate Agrobacterium?

I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally...

18 October 2023 4,749 3 View

Why do I get an arc when I electroporate Agrobacterium?

I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally...

01 January 1970 4,816 1 View