Can we attribute that protein coatings prevents the IONPs dissociation once the particle enters inside the cellular environment, or is this the function of serum proteins available in the body, or is it something else?
Pristine nano-particles cause what is known as "oxidative stress" whereas in surfactant coated NPs, this phenomenon is minimized..
Read this paper: http://pubs.acs.org/doi/abs/10.1021/tx800064j
well thanks Sridhar Vadahanambi........for you valuable comments, In surfactant coated magnetic iron oxide Nanoparticle this phenomena is minimised but after getting dissociated inside the cells either in lysosomes or in the intracellular compartment it will finally come in the Fe2+ and Fe3+ forms which will then react with H2O2 present and undergoe fenton reaction and cause cytotoxicity ......so what is the actual reason which is preventing the toxicity of cells when we use protein Coated magnetic nanoparticle?
Hello Purva,
I am also working on the same research. There are different mechanism by which IONs causes the generation of toxic ROS like agglomeration and fenton reaction as you said. We use surfactant to form the thin layer around the NP so that they will not agglomerate. Also this coating helps to minimize the re-activity of the IONP resulting in less generation of ROS. I think this might help. Please go through the link. Thanks
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215220/
@Purva...
Yes, SPIONs will finally yield Fe3+ or Fe2+ ions.. but in your case this process is delayed, due to the presence of surfactant or protein protective layer...
@ Sridhar, well i agree to what you have explained, but then i have some of the comparison with the commercially available magnetic nanoparticles also which they claim to be BARE but are not, they are coated with some or the other polymer either organic or inorganic, even then they are showing toxicity where protein coated magnetic particle are showing no toxicity, how can we explain this fact......
@sachin , well i have already gone through this review article, but since you are also working on the same parameters have you also undergone such experience of toxicity in relation with the coating, what are youe experiences and how will you explain this fact that coating prevents cytotoxicity.....
@Purva....
Perhaps your commercial SPIONs are stabilized or capped with surfactants.. test them with FTIR...
@purav in my case i am using a gold coat which prevent the conversion of ferrous to ferric ions so there is no ROS and ultimatelly no toxicity. I think in yor case these will be partial hydrolysis of coat due to which the rate of reaction is slowing.
@Sachin...do you have paper explaining this fact of partial hydrolysis.....
@purva I dont have any paper concerning that but I am damn sure that there is some coating around the IONP preventing ferrous to ferric conversion. Thanks
wish you good luck with your research.
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology
- Nanoparticle Research