Why DNA skein and DNA pellet were not seen during salting out DNA extraction method?

21 September 2019 1 3K Report

I use salting out DNA extraction methods . But recently after adding the ethanol we couldn't saw the DNA skein and DNA pellet . Finally the results of nano drop indicated that extracted DNA have 100 to 500 ng/microliter and OD 1.7 to 1.9 .

How can i solve this problem?

Anooj E.S

It may be denatured

Badges
Science topic

Similar topics
Chemistry
Nanotechnology
Nano

More Saeid Afshar's questions See All

Small displacement measurement in vacuum?

Hello, I have a question. there is a circular thin foil which vibrates in vacuum, the foil restricted and moves up and down. it is like a diaphragm between two pipes . both sides are in vacuum but...

18 February 2021 8,075 3 View

I have a problem with sensible heat flux (H) iteration process in Sebal model?

In each iteration, I get the same value of H for different values of aerodynamic resistance and the temperature difference between two heights. Do the results make sense? Is it necessary to adjust...

07 February 2021 8,953 2 View

What is the best book to teach entrepreneurship to primary students?

07 September 2020 6,757 3 View

Which algorithm (Sobel, Perwitt, Roberts, Canny and LOG algorithm) is more capable of finding noisy image edges?

The edge detection algorithms are designed to respond to sharp edges which can be caused by noisy pixels. In image analysis edge detection plays an important role and it is one of the traditional...

05 August 2020 2,543 1 View

Internal pressure of CT scan x-ray vacuum insert ?

Hello,, Dose anyone know the internal pressure of x-ray vacuum insert used in CT scan machines? Thank you

03 August 2020 8,748 2 View

Which source do you advise to learn Ansys Fluent?

Dear Colleagues, I am currently working on a new project which needs to be modeled by ANSYS FLUENT. As you know, there are many sources including tutorials, online courses, books, etc. However, I...

11 July 2020 2,344 10 View

What is the best way of Evaluation Performance for Multiclass Data?

For classification problems, classifier performance is typically defined according to the confusion matrix associated with the classifier. Based on the entries of the matrix, it is possible to...

19 May 2020 3,472 4 View

Best EEG dataset for sleep stage classification?

I am looking for a best dataset with signals of EEG that is used for sleep stage classification.

12 May 2020 5,935 3 View

What are the public biosignals (ECG, etc) datasets of COVID-19 patients?

I am researching QOVI-19 and need a database that contains biosignal data such as electrocardiograms. Is there any dataset?

12 May 2020 9,274 3 View

How can I download FDA approved drugs from zinc database ?

Recently I want to download the FDA approved drugs but unfortunately I couldn't retrieve data correctly . How can I download this data in SDF format?

28 March 2020 3,785 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View