Statistical analysis of fish densities between contiguous river reaches?

I have fish density data (n per 100m sq) for fish populations in three river systems. Each river has a site immediately above a effluent discharge, a site immediately below it, and two further...

30 June 2019 4,397 8 View

Good antibody for oligodendrocyte precursors that is not a rabbit polyclonal?

Hi, I really need a good antibody to do immunochemistry. It should visualize oligodendrocyte precursors. The most important. It should work on mouse brain or CNS cells and it cannot be a rabbit...

10 October 2018 7,363 4 View

What is different between RM or Ordinary one-way ANOVA & What kind of experiment is suitable for each of them?

Dear All, I just switch from SPSS to Graphpad prism 6. In analisys, there is option to select experimental design between RM (repeated measure) and Ordinary (no pairing) for one-way ANOVA. What is...

24 February 2016 3,486 3 View

Is Fas ligand's western blot signal strong in general cell line?

23 February 2016 4,669 2 View

How should I select western blot band to measure if getting multiple target protein bands? any online database to look for?

Dear All, I am real newbie in protein field. I would like to ask you a few question regarding to select band to measure in western blot. In case that blocking is fine and I use single primary...

14 February 2016 6,246 7 View

How should I solve blochy/uneven background problem on western blot?

Dear All, In my western blot experiment, I have problem with blotchy blackground (like in attachecd picture) on the blot, which usaully appear when I expose low signal band for 15-30 min or more...

09 January 2016 7,076 6 View

Could you recommend a good recipe for 5X or more X Laemmli buffer?

Dear All, I try to make 5x Laemmli buffer (10% SDS, 50% glycerol, 25% 2-mercaptoethanol, 0.02% bromphenol blue and 0.3125 M Tris HCl, pH approx. 6.8.) adapting from Sigma's 2X Laemmli buffer, but...

20 November 2015 6,144 4 View

How can I improve signal on low amount target protein (45-48kda) in PVDF-wet transfer, Western blot?

Dear All, My target protein has two isoforms, one is at 60kda, which give strong WB band, however another is at 45-48kda, which has very weak WB signal, even after 1hr exposure with GE's Amersham...

19 November 2015 5,940 1 View

With or without plastic wrap/cover: Which is best solution to expose chemiluminescent western blot by using CCD camera?

Hi guys, From my expirence there are pros and cons of with or without plastic sheet cover: 1. Plastic sheet cover sheet is good to keep the blot wet, good for long time exposure, but it will have...

26 October 2015 5,907 4 View

Is it ok to select all lanes in one rectangle and draw vertical line to seperate each lane for gel image analysis using ImageJ?

Hello, For using ImageJ to analyse gel image, normally I select each lane for each rectangle/box. but some guides suggest to draw a rectangle around all the lanes of gel image in one box and then...

06 October 2015 8,007 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View