Statistical analysis of fish densities between contiguous river reaches?

I have fish density data (n per 100m sq) for fish populations in three river systems. Each river has a site immediately above a effluent discharge, a site immediately below it, and two further...

30 June 2019 4,397 8 View

What is different between RM or Ordinary one-way ANOVA & What kind of experiment is suitable for each of them?

Dear All, I just switch from SPSS to Graphpad prism 6. In analisys, there is option to select experimental design between RM (repeated measure) and Ordinary (no pairing) for one-way ANOVA. What is...

24 February 2016 3,486 3 View

Is Fas ligand's western blot signal strong in general cell line?

23 February 2016 4,669 2 View

How should I select western blot band to measure if getting multiple target protein bands? any online database to look for?

Dear All, I am real newbie in protein field. I would like to ask you a few question regarding to select band to measure in western blot. In case that blocking is fine and I use single primary...

14 February 2016 6,246 7 View

How should I solve blochy/uneven background problem on western blot?

Dear All, In my western blot experiment, I have problem with blotchy blackground (like in attachecd picture) on the blot, which usaully appear when I expose low signal band for 15-30 min or more...

09 January 2016 7,076 6 View

Could you recommend a good recipe for 5X or more X Laemmli buffer?

Dear All, I try to make 5x Laemmli buffer (10% SDS, 50% glycerol, 25% 2-mercaptoethanol, 0.02% bromphenol blue and 0.3125 M Tris HCl, pH approx. 6.8.) adapting from Sigma's 2X Laemmli buffer, but...

20 November 2015 6,144 4 View

How can I improve signal on low amount target protein (45-48kda) in PVDF-wet transfer, Western blot?

Dear All, My target protein has two isoforms, one is at 60kda, which give strong WB band, however another is at 45-48kda, which has very weak WB signal, even after 1hr exposure with GE's Amersham...

19 November 2015 5,940 1 View

With or without plastic wrap/cover: Which is best solution to expose chemiluminescent western blot by using CCD camera?

Hi guys, From my expirence there are pros and cons of with or without plastic sheet cover: 1. Plastic sheet cover sheet is good to keep the blot wet, good for long time exposure, but it will have...

26 October 2015 5,907 4 View

Is it ok to select all lanes in one rectangle and draw vertical line to seperate each lane for gel image analysis using ImageJ?

Hello, For using ImageJ to analyse gel image, normally I select each lane for each rectangle/box. but some guides suggest to draw a rectangle around all the lanes of gel image in one box and then...

06 October 2015 8,007 5 View

What is the ratio of 10%FBS culture media to 0.25%trypsin-EDTA to inactivate/neurtrilize trypsinization in cell culture?

Dear All, What is the ratio of FBS to trypsin to inactivate/neurtrilize trypsinization in cell culture? In some protocol, the ratio of 10%FBS media to 0.25%trypsin-EDTA is 1:1 or 2:1. ATCC guide...

02 September 2015 3,704 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View