In Asia most of the clinic has been using blood agar (ex BAP, Chocolate and CDC) from BD as the main supplier.
Their quality are always showing good, the color is so brilliant red compared to other local manufacture, the colony of P.aeruginosa is so big and moving and swarming so fast than others.
Even we use the BD TSA powder to make Blood agar by ourselves, the color and the quality from us still cannot compete against BD.
We doubtfully there are lots of difference in blood agar formula.
Could anyone teach us or give us a hint to enhance our agar?
Blood agar is a general purpose enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties.
** It is typically Tryptic Soy Agar or Columbia Agar base with 5% Sheep blood.
** Sheep blood has been preffered source in the Blood agar due to the fact that sheep RBCs are most sensitive to the the hemolytic toxins released by bacterial cells thus causing hemolytic zones around the colonies over the period of time.
** Also, agar prepared by using nutrient agar with 5% of human blood is not recommended, partly because of the safety risk to laboratory personnel, but mainly because it is said to result in poor bacterial isolation rates, although there are few published data to support this.
In general it's an enrichment media so bacterial growth will be higher than other media...
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