ZnO is known for antibacterial activity. Different methods have been adopted for the assessment and investigation of antibacterial activity, hence, it is important that which methods you are following.
I want to add some points to Mr. Sarkar answer, Yes he is right. ZnO nanoparticles are known for their antibacterial properties, But for application like antibacterial activity; shape, size and uniform morphology of the generated nanoparticles also plays a critical role..........As you know that physical parameters like surface area of nanoparticle is also a key factor in its utility in various field even in antibacterial.........so just see your morphology and size distribution of your generated nanoparticles........Bcoz different shape and size of same nano material shows different antibacterial properties.......so i think in your case morphology of the generated nanoparticle is the problem..........