Hi,
I'm using Quiagen DNeasy Plant Mini Kit with mortar and pestle for my fungal gDNA extractions, but sometimes there are two bands on the gel. The samples are intended to be sequenced after extraction.a
It doens't look like the normal degradation, which looks like a smear. One band is located above 10kb and one is located at 6kb. Do you have so tips for me?
Thanks in advance!
Hi,
this thing is very strange. I suggest you to run the sample in the gel 2% and try to separate very well the two bands. After you cut the band and try to obtain a separate DNA extraction process and after you will try to sequence them.
I have a question. Did you make the gDNA extraction by pure culture or soil?
I will try to run the 2% gel and sequence it to see what happend. Since the gDNA is isolated from mutants, could it be that there was some break off at the chromosome? Sequencing afterwards sounds good to see what happend.
I isolate from pure stationary liquid culture grown overnight. I take only the young mycelia pellicle for isolation.
But the mutation how much increase or decrease the size of the genome?Because is possible that you are in presence of mutants or not?
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