Why is the GFP expressed from my viral vector so weak?

I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...

19 October 2020 2,863 3 View

Is Intechopen a reputable Publisher?

I have received numerous invites from IntechOpen to contribute to their books. Can anyone assist is this publisher reputable?

08 June 2020 4,217 3 View

How to perfom Unconflated Multilevel analysis?

I want to use Unconflated Multilevel Modeling (UMM) to test a 1-1-1 model in which the effects of the mediating and predictive variables can also be on level 2. Because of a small sample size (164...

24 December 2019 8,603 3 View

Nicotiana benthamiana growth chamber conditions?

UPDATE: SEE MY LAST COMMENT FOR SOLUTION! I'm trying to figure out the best conditions to grow my N. benthamiana plants in a growth chamber. I will be Agro infiltrating them later, around 4...

11 November 2019 6,018 10 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Plant histology stains?

Hello, I'm looking for plant histology methods for use on traditional microscopes (we have a UV lamp) instead of confocal laser (too expensive for our lab to use) that would allow me to...

18 November 2018 7,647 1 View

How to interpret the following mediation outcome (regression based)?

In my research I hypothised that team learning mediates between transformational leadership and innovative work behavior. To analyse the relations I use HLM 7. I found a significant positive...

25 January 2018 9,913 4 View

Has anyone succesfully expressed Gq DREADDS in rats?

My lab is interested in using DREADDS (Gq) targeting excitatory neurons in rats, specifically Long Evans. Unfortunately, we have not been able to successfully express Gq in cell culture (mice) or...

22 June 2016 8,233 1 View

How long can formalin-methanol fixed cells be in good condition for sorting and further RNA analysis?

18 February 2016 2,358 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How to calculate stress at the cross section of spherical particles within a matrix?

Hi all, I am currently working on some background calculations before I start a new project related to metal matrix particulate composites. I am trying to find a way to calculate the stress at...

19 February 2021 8,503 3 View

How can I generate a population with a fixed correlation coefficient in R?

I want to generate an artificial population using two variables (say x & y) with a fixed correlation between x & y. Which package/command in R software can help in this?

18 January 2021 2,545 9 View

What causes the temperature variation of Carbon/PAEK composite sample while heating with AC current at certain voltage in a number of cycles/one time?

It should be either decrease or increase for nth time during heat supply. Is there any possibility of irregular temperature sequences as shown in figure. In that picture the highest temperature...

12 January 2021 4,390 4 View

R package for multivariate dispersion as a measure of beta diversity?

Hello, everyone: I've been reading some old and new papers on how to compute beta diversity using incidence or abundance data from several assemblages/communities. For my data, I am interested...

03 January 2021 9,293 1 View

How can I build one phylogenetic tree for an arthropod community when I had known their scientific names?

I am working on the phylogenetic analysis for communities of plants and arthropods. A standardized protocol of building phylogenetic tree for plant species list that was widely used in studies....

29 December 2020 181 3 View

How to do qualitative field research in a hostile environment?

It is much said about doing qualitative research and field study. I'm curious about your hints/experiences in doing ethnography in hostile environment touching sensitive topics (marginalized...

30 November 2020 4,151 14 View

What are the best binder which can be used for deposition of MXene on FTO?

I would like to coat MXene (Ti3C2Tx) on FTO glass substrate using doctor blade deposition technique, for which i think a binder is essential for the adhesion of MXene onto the FTO. The post...

28 November 2020 9,360 3 View

I am simulating a three-dimensional panel with a mortar coating, i am looking for the mechanical characteristics of a mortar for abaqus, please help?

I am simulating a three-dimensional panel with a mortar coating, my partner and I are looking for the mechanical characteristics of a mortar with a compressive strength of 180 kg / cm2 to define...

11 November 2020 9,001 1 View

Why is the impedance of ECoG electrodes tested at a frequency range from 1 Hz -100 kHz?

I am testing the impedance of a material that will be used for ECoG electrodes. I was told to test it at a frequency range from 1 Hz - 100 kHz but why is this range used? I wasnt able to find a...

08 November 2020 8,089 3 View