I did qPCR for my bacterial cDNA samples. But in the end, I got a strange result. Here is attached the pictures of the amplification curve and PCR conditions. Thank you
Have you ran these primers or samples before? It looks like your melting curves. Your annealing temp is a bit low. Generally we have good RT-PCR reaction if we run at 60 then we evaluate the melting curves and if they do not look uniform, we bump it up to 65. Generally this always works.