Graphite can be intercalated with a number of compounds including halides, alkali metals, halogens etc. Yet most of the literature points towards use of graphite-acid intercalated compounds.
Hello all, I am over-expressing acid sphingomyelinase enzyme in HeLa cells. Theenzyme shows expression on western blot but I do not have any activity in in vitro assays. I have checked the...
06 July 2023 365 3 View
Hello, I am using the following tutorial for microRNA profiling: https://drmirdeep.github.io/mirdeep2_tutorial.html I was able to follow it until the patching step which is: bash...
31 October 2022 6,779 0 View
Does anyone have experience growing single cell clones of MCF-10A.I have generated gene knockouts and need to pick single cell clones from these cell lines. It is challenging as these cells are...
28 April 2021 1,857 2 View
I am using MCF10A cell lines. I see different sensitivity to Blasticidin of my control cells (not infected with lentivirus) vs cells infected with lentivirus. I thought this is a good thing as My...
25 February 2021 7,066 1 View
I am making CRISPR knockouts in MCF10A cells and Blasticidin is used as a selection marker. When I add Blasticidin, some cells die which is expected as they haven't received my sgRNA. However,...
11 December 2020 4,710 2 View
I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...
03 August 2020 3,001 4 View
I have added anti- CD28/anti-CD3 beads to in vitro culture of murine T cells. Some of the cells have increased in size which indicates their activation. However, is there a method to quantify the...
08 August 2019 3,538 7 View
Aim is damaged part detection (i.e. scratch, dent, holes, pits, etc.): e.g. Automotive/Industrial part stamping quality inspection, Rolled sheet metal surface inspection, Pit/hole detection in...
06 May 2018 1,401 1 View
I have all required data (structural data for the phases, XRD input file). I am just unable to locate the option which will allow me to know the percentage composition of the sample.
05 June 2017 3,721 1 View
I wish to intercalate very fine graphite particles through a mixture of concentrated H2SO4 and HNO3. Normally, small particle size results in greater degree of intercalation. Is it possible for...
16 May 2017 5,703 2 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?
03 August 2024 4,624 3 View
I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...
03 August 2024 5,384 0 View
Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...
03 August 2024 5,358 0 View
Is this circle one of the rings?
03 August 2024 5,988 0 View