As opposed to saline, phospate buffered saline maintains a constant pH (~7.4). Additionally, its ion concentration and osmolarity also matches that of the fluid inside the cells.
Saline maintain osmotic balance between internal and external environment of cell. Phosphate buffer maintain physiological pH. Phosphate ion is known to all cells, so it is wise to use physiological buffer made of phosphate ion to keep cell healthy
You can also do the same with Tris buffer saline, which is just to maintain the pH and saline (salt concentration) conditions both in vivo and in vitro environments.
In cell culture during spilitting PBS washing is needed to remove the serum of media so that trypsin will able to detach the cells from plate other wise serum can inactive the trypsin.
This is just to avoid colour quenching in fluorescent and colourimetry based assays and to avoid chemical quenching in chemiluminescent assays. If you are not considered about these two factors, you can wash your cells with serum free media, instead of PBS.
For the properties of PBS just go through the above answers. Am just adding to them.
Basically, I think your question has been sufficiently answered. However, this link may add one or 2 points to buttress what has already been said.
http://wiki.answers.com/Q/What_is_the_composition_of_physiological_saline
I think because the pH value of buffer neutral (7) not affect on bacteria during washing process in relation to diffusion and osmosis processes in cell .
as other frends mentioned above its for maintaining the pH and osmolarity that matches those of human body. Plus, it is used with and without ca2++, mg2++ for different conditions which is well explained in this minireview i've attached here.
What's new in it ? same was mentioned by most of the friends. Question of ca/Mg is not raised in the issue
I added drug to the cell, for the cell cycle experiment. But Unfortunately I forgot to wash the cell with PBS. Is it affect the action of drug ?
PBS has many uses because it is isotonic and non-toxic to most cells. The pH of PBS is set to be 7 to 7.6, so it can maintain the constant pH of the cells.
PBS is an isotonic and non-toxic solution which keeps tissue intact preventing them from rupturing. Similarly, it provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
ok so what happen if the PH change?.
is the PBS used to disengage cells clumping?
what is the good reagent used to remove all cells from the wall of the Eppendorf, because I want to get cells as much as I can?
the cells will not be disrupted i.e the DNA-protein interaction will remain intact since it has a physiological pH. You can use PBS while washing cells for site identification on a gene.
All important points have been mentioned here. I had so many of such questions when I started on my PhD but I came to learn most through literature. However, could someone help to clarify on the optimum molarity for PBS and the 1x or 10xPBS. Kind regards.
Question about the PBS:
-I would like to do DNA extraction with blood pellet but my sample is very low so I can't transfer it to another vials for extraction. So I wonder if I can add the PBS to the sample befor the extraction! I am using ZYMO KIT with the PK Digest Buffer.
I washed my cells with incomplete media instead of PBS and just removed media, and provided fresh media. I see after 24 hours no cells are attached. What could be the reason?
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