Antioxidant activity of Schiff,s bases normally taken in methanol in which both reagent (DPPH) and the compounds are soluble clearly..

Hi Dr Anjali Krishna G . I think because it is more stable in methanol.

Madhukar Baburao Deshmukh Logically, the antioxidant activity should be measured under physiological conditions. Neither DPPH nor methanol fit this requirement.

Aref Wazwaz What is more stable in methanol?

Anjali Krishna G "Also why buffer solution is not taken as a solvent medium?" You ask the right question. Use ABTS assay as a starting point. Don't forget that metal complexes might have pro-oxidant activity

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Schiff bases and their complexes are versatile compounds prepared from the condensation of primary amines with carbonyl groups. Schiff bases are very important compounds in various fields, especially in medicinal and pharmaceutical fields because of their wide spectrum of biological activities. The activity is usually increased by complexion therefore understanding the properties of both ligands and metals can lead to the synthesis of highly active compounds Anjali Krishna G

Dear Anjali Krishna G thank you for posting this very interesting technical question on RG. To the best of my knowledge the use of methanol when measuring the antioxidant activity of Schiff bases is just a matter of solubility and convenience. Methanol is cheap, and the solubility of DPPH in methanol is quite reasonable (10 mg / mL according to Wikipedia). However, other lower alcohols such as ethanol and isopropyl alcohol can also be used as solvent. In this context please have a look at the answers given to the following closely related question which has been asked earlier on RG:

Can we use some other solvent for DPPH assay instead of methanol?

https://www.researchgate.net/post/Can-we-use-some-other-solvent-for-DPPH-assay-instead-of-methanol

(28 answers)

In addition, there are plenty of relevant literature references about this research topic available. As a typical example, please check the following article which is freely available as public full text:

Antioxidant and Cytotoxic Activity Studies in Series of Higher Amino Acid Schiff Bases

https://dergipark.org.tr/en/download/article-file/1148800

(please see the attached pdf file)

Good luck with your work and best wishes, Frank Edelmann

Thanks about this question.

I agree with frank Answer.

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