Hello
I wanted to know why and when should Cytosine(C) or Tymidine (T) in Probe sequence change to pdC and pdU in probe synthesis ? and does this modification could occur in primers too?
Thank you
Hi Dear Researchers, Could anyone please provide me with some information about the HPLC column, specifically the “Purospher STAR RP-18 LiChroCART Cartridge”? I would like to know if it is...
25 June 2024 9,523 1 View
In Iran, there is informal co-creation of curriculum in higher education; But formally, no What do we need to implement curriculum co-creation in higher education?
28 May 2024 3,019 14 View
During the publishing of an article, the reviewers have asked me for the complete data file, my calculation information, and the example simulation input file. I have no experience with this. What...
14 April 2024 8,825 2 View
We are looking to investigate changes in soil enzyme activity under the influence of different management practices in Hyrcanian forests. I would be grateful if someone could guide me in measuring...
14 December 2023 3,905 2 View
(I'm not sure about how much water was added)
15 July 2023 9,457 6 View
description is in the file attached.
10 July 2023 2,238 5 View
Hi all! I have been trying for a while to clone two fragments in a plasmid for expression of long hairpin dsRNA. My strategy was to insert the sense fragment first and then the antisense on the...
05 June 2022 6,774 1 View
I used Central Composite Design to optimize effective factors for a typical determination. Now, as shown in attached Figure, in 3D response surface plots, response (peak area) is started from...
01 April 2022 8,672 0 View
Hello, How can I use all cores of my system for Sorption calculation in Material Studio software? Is it possible? Thank you!
28 December 2021 2,062 3 View
How should I run MD on a protein with a unusual amino acid (FGL or formyl glycine)? I would appreciate it if anyone can help to solve it.
06 June 2021 9,996 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,667 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,113 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,056 3 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,672 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 840 2 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 905 2 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View